The interaction mode between DNA and salen-Co(III) N,N’-dipyridoxyl (1,4-butanediamine) Shiff-base complex

نویسنده:
, , , , , , , , , , ,
سال
: 2010
چکیده: In this work, we studied the interaction of new salen-co(III) of

N,N\\\\\\'-dipyridoxyl (1,4-butanediamine) Schiff-base complex with DNA by

melthing temperature, fluorescence spectrometry and gel

electrophoresis techniques. This salen-Co(III) complex shows increase

in melting temperature when bound to native calf thymus-DNA (CTDNA).

The intersection point of the binding isotherm indicated a

binding site size of 3 bp per bound complex molecule in Tris–HCl

buffer. Upon adding the new salen-Co(III), the electrophoretic mobility

of pTZ57R DNA plasmid becomes slower for both super coiled and

open circular forms without any structural changes in DNA. The

experimental results showed that the salen-Co(III) complex bound to

DNA by intercalative mode.In this work, we studied the interaction of new salen-co(III) of

N,N
کلیدواژه(گان): Schiff bases,anticancer,melting temperature,structural change
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