The interaction mode between DNA and salen-Co(III) N,N’-dipyridoxyl (1,4-butanediamine) Shiff-base complex
Author:
, , , , , , , , , , ,Year
: 2010
Abstract: In this work, we studied the interaction of new salen-co(III) of
N,N\\\\\\'-dipyridoxyl (1,4-butanediamine) Schiff-base complex with DNA by
melthing temperature, fluorescence spectrometry and gel
electrophoresis techniques. This salen-Co(III) complex shows increase
in melting temperature when bound to native calf thymus-DNA (CTDNA).
The intersection point of the binding isotherm indicated a
binding site size of 3 bp per bound complex molecule in Tris–HCl
buffer. Upon adding the new salen-Co(III), the electrophoretic mobility
of pTZ57R DNA plasmid becomes slower for both super coiled and
open circular forms without any structural changes in DNA. The
experimental results showed that the salen-Co(III) complex bound to
DNA by intercalative mode.In this work, we studied the interaction of new salen-co(III) of
N,N
N,N\\\\\\'-dipyridoxyl (1,4-butanediamine) Schiff-base complex with DNA by
melthing temperature, fluorescence spectrometry and gel
electrophoresis techniques. This salen-Co(III) complex shows increase
in melting temperature when bound to native calf thymus-DNA (CTDNA).
The intersection point of the binding isotherm indicated a
binding site size of 3 bp per bound complex molecule in Tris–HCl
buffer. Upon adding the new salen-Co(III), the electrophoretic mobility
of pTZ57R DNA plasmid becomes slower for both super coiled and
open circular forms without any structural changes in DNA. The
experimental results showed that the salen-Co(III) complex bound to
DNA by intercalative mode.In this work, we studied the interaction of new salen-co(III) of
N,N
Keyword(s): Schiff bases,anticancer,melting temperature,structural change
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The interaction mode between DNA and salen-Co(III) N,N’-dipyridoxyl (1,4-butanediamine) Shiff-base complex
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| contributor author | زهرا مشهدی خوشخو | en |
| contributor author | محمدرضا حسین دخت | en |
| contributor author | راضیه جلال | en |
| contributor author | حسین اشتیاق حسینی | en |
| contributor author | سیدحمید میرطباطبایی | en |
| contributor author | مسعود میرزائی شهرابی | en |
| contributor author | Zahra Mashhadi Khoshkhoo | fa |
| contributor author | Mohammad Reza Housaindokht | fa |
| contributor author | Razieh Jalal | fa |
| contributor author | Hossein Eshtiagh Hosseini | fa |
| contributor author | sayyed hamid mir tabatabae | fa |
| contributor author | Masoud Mirzaei Shahrabi | fa |
| date accessioned | 2020-06-06T14:03:30Z | |
| date available | 2020-06-06T14:03:30Z | |
| date copyright | 2/24/2010 | |
| date issued | 2010 | |
| identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3380653 | |
| description abstract | In this work, we studied the interaction of new salen-co(III) of N,N\\\\\\'-dipyridoxyl (1,4-butanediamine) Schiff-base complex with DNA by melthing temperature, fluorescence spectrometry and gel electrophoresis techniques. This salen-Co(III) complex shows increase in melting temperature when bound to native calf thymus-DNA (CTDNA). The intersection point of the binding isotherm indicated a binding site size of 3 bp per bound complex molecule in Tris–HCl buffer. Upon adding the new salen-Co(III), the electrophoretic mobility of pTZ57R DNA plasmid becomes slower for both super coiled and open circular forms without any structural changes in DNA. The experimental results showed that the salen-Co(III) complex bound to DNA by intercalative mode.In this work, we studied the interaction of new salen-co(III) of N,N | en |
| language | English | |
| title | The interaction mode between DNA and salen-Co(III) N,N’-dipyridoxyl (1,4-butanediamine) Shiff-base complex | en |
| type | Conference Paper | |
| contenttype | External Fulltext | |
| subject keywords | Schiff bases | en |
| subject keywords | anticancer | en |
| subject keywords | melting temperature | en |
| subject keywords | structural change | en |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1024417.html | |
| conference title | 9th Iran Biophysical Chemistry Conference | en |
| conference location | تهران | fa |
| identifier articleid | 1024417 |