Transient expression of CCL21as recombinant protein in tomato

Abstract

The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine<br><br>ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for<br><br>anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct<br><br>of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agroinfiltrated<br><br>into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was<br><br>confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by<br><br>western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed<br><br>leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic<br><br>function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein.