Transient expression of CCL21as recombinant protein in tomato
نویسنده:
, , , , , , ,سال
: 2018
چکیده: The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine
ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for
anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct
of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agroinfiltrated
into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was
confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by
western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed
leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic
function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein.
ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for
anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct
of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agroinfiltrated
into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was
confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by
western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed
leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic
function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein.
کلیدواژه(گان): Recombinant vaccine
Agroinfiltration
Tomato
Transient gene expression
Migration
Scratch assay
کالکشن
:
-
آمار بازدید
Transient expression of CCL21as recombinant protein in tomato
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| contributor author | ماریا بیهقی | en |
| contributor author | سیدحسن مرعشی | en |
| contributor author | عبدالرضا باقری | en |
| contributor author | مجتبی سنکیان | en |
| contributor author | maria beihaghi | fa |
| contributor author | Hassan Marashi | fa |
| contributor author | Abdolreza Bagheri | fa |
| contributor author | mojtaba sankian | fa |
| date accessioned | 2020-06-06T13:37:55Z | |
| date available | 2020-06-06T13:37:55Z | |
| date issued | 2018 | |
| identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3362741 | |
| description abstract | The main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agroinfiltrated into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein. | en |
| language | English | |
| title | Transient expression of CCL21as recombinant protein in tomato | en |
| type | Journal Paper | |
| contenttype | External Fulltext | |
| subject keywords | Recombinant vaccine Agroinfiltration Tomato Transient gene expression Migration Scratch assay | en |
| journal title | Biotechnology Reports | fa |
| pages | 15-Oct | |
| journal volume | 5 | |
| journal issue | 17 | |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1065965.html | |
| identifier articleid | 1065965 |