Studies on interaction between propranolol and HSA in presence and absence of magnetic field

Abstract

Human serum albumin (HSA) is the most abundant carrier protein of<br><br>the blood with a high affinity for a wide range of metabolites and drugs<br><br>and have many physiological functions. Propranolol, 1-[isopropylamino-<br><br>3-[1-naphthyloxy]-2-propanol], is a adrenoceptor antagonist (-blocker),<br><br>which is widely used in the treatment of several diseases such as<br><br>cardiac arrhythmia, angina pectoris, sinus tachycardia, thyrotoxicosis,<br><br>hypertrophic subaortic stenosis and hypertension. The effects of static<br><br>magnetic fields (SMFs) on biological systems have been a topic of<br><br>considerable interest for last two decades. The increasing production of<br><br>electric (EMFs) and magnetic fields (MFs) due to the increasing use of<br><br>electronic devices in homes and work places, is encouraging studies on<br><br>the influences of magnetic fields on living organisms with a goal to<br><br>protect better human health against their probable unfavorable effects.<br><br>In this paper, the interaction between propranolol HSA and in<br><br>physiological buffer (pH 7.4) was investigated by UV and fluorescence<br><br>absorption spectroscopy. In order to understand the effect of magnetic<br><br>field, all experiments were also done in the presence of magnetic field<br><br>(3 mT); and the binding parameters were determined for both cases.<br><br>The Hill equation was employed to analyze the data obtained, for<br><br>determining of the binding capacity, g, binding constant, K, and Hill<br><br>constant, nH,.<br><br>The results showed that the values of g and nH increased slightly<br><br>in the presence of magnetic field, and the binding constant in both<br><br>method, uv and fluorescence spectroscopy, was decreased in the<br><br>magnetic field exposed situation.