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RISبررسی میزان آلودگی پنیر عرضه شده در مشهد به باکتری لیستریا مونوسیتوژنز با استفاده از روش Multiplex-PCR
هدف از این مطالعه تعیین میزان آلودگی نمونه های پنیر عرضه شده در مشهد به باکتری لیستریا مونوسایتوژنز با استفاده از روش کشت در محیط انتخابی و تایید تشخیص به روش Multiplex-PCR می باشد. دراین بررسی تعداد 110 نمونه پنیر سنتی ...
Prevalence of salmonella enteritidis contamination in broiler carcasses slaughtered in a poultry abattoir of Mashhad using multiplex-PCR
: In this study 14% of poultry carcasses were determined as contaminated with salmonella spp. And 6% as contaminated with a salmonalla enteritidis. It is pertinent to use m=PCR in order to provide a more accurate profile of the prevalence of salmonella spp...
Isolation, Identification and Virulence Gene Profiling of Escherichia coli O157:H7 in Retail Doner Kebabs, Iran
the presence of virulence factors were performed. Specific primers for cH7 and
rfbO157 genes in the first and for stx1 and stx2 genes in the second m-PCR were
used. The third m-PCR performed using specific primers for eaeA and Ehly...
Prevalence of Campylobacter jejuni and Campylobacter coli isolated from raw milk samples in Mashhad
. The suspected colonies were isolated on sheep blood agar and tested for morphology, motility, gram staining, biochemical properties and hippurate hydrolysis activity. Then multiplex PCR assay (m-PCR) with three sets of primers was employed for concurrent...
Isolation and identification of Campylobacter spp and Campylobacter coli from poultry carcasses by conventional and multiplex PCR method in Mashhad, Iran
media, the suspected colonies were isolated on sheep blood agar and tested for morphology, motility and gram staining. Biochemical tests and hippurate hydrolysis activity were also performed. Concurrently, a multiplex PCR assay (mPCR) with two sets...
Isolation and Identification of Campylobacter jejuni from Bulk Tank Milk in Mashhad-Iran
and tested for morphology, motility, gram staining, biochemical properties and hippurate hydrolysis activity. For comparison, a multiplex PCR assay (m-PCR) from suspected colonies with two sets of primers was employed for concurrent identification...
Using multiplex-PCR assay in identification of Escherichia coli O157: H7 isolated from hamburger samples in Mashhad, Iran
by biochemical tests as Escherichia coli and then employed for multiplex-PCR assay, using primers specific for O157 and H7 antigens gene. The m-PCR assay employed in this study may be a possible alternative to immunological assays which detects somatic...
Identification of Escherichia coli O157:H7 Isolated from Cattle Carcasses in Mashhad Abattoir by Multiplex PCR
non sorbitol fermenting (NSF) colonies were confirmed
by biochemical tests as Escherichia coli and then employed for multiplex-PCR (m-PCR) assay. In the first m-
PCR assay, primers specific for O157 and H7 antigens gene were used. A total...
Isolation of Escherichia coli O157:H7 from cattle carcasses in Mashhad abattoir using multiplex PCR
Escherichia coli O157:H7. The second multiplex PCR assay showed that two isolates were harboring the Shiga toxin (stx2) producing
gene. The m-PCR assays employed in this study might be considered as a possible alternative for immunological assays detecting...
Quantification of Escherichia coli O157:H7 in milk by most probable number –polymerase chain reaction (MPN-PCR) method
was performed by DNA extraction from tubes showing turbidity and performing
multiplex-PCR (m-PCR) using primers specific for O157 and H7 antigens gene. This MPN-PCR proved to
be a rapid and reliable method for enumerating E. coli O157:H7 in milk...