Isolation, Identification and Virulence Gene Profiling of Escherichia coli O157:H7 in Retail Doner Kebabs, Iran
نویسنده:
, , , , , , , , ,سال
: 2013
چکیده: Most illnesses caused by Escherichia coli O157:H7 have been associated with eating
undercooked, contaminated meat. In this study, a number of 200 cooked beef
doner kebab samples from different restaurants across cities of Tehran and
Mashhad were analyzed. After enrichment, selective plating and biochemical
tests, the DNA from suspected colonies were extracted. Three multiplex polymerase
chain reaction (PCR) assays to confirm the isolates as E. coli O157:H7 and
the presence of virulence factors were performed. Specific primers for cH7 and
rfbO157 genes in the first and for stx1 and stx2 genes in the second m-PCR were
used. The third m-PCR performed using specific primers for eaeA and EhlyA
genes. From 25 nonsorbitol-fermenting isolates, 21 isolates confirmed as E. coli
O157:H7. The results of third m-PCR showed 12 isolates harboring the EhlyA
gene, three isolates the eaeA gene and three isolates harboring both the EhlyA and
eaeA genes.
PRACTICAL APPLICATIONS
The presence of Escherichia coli O157:H7 in cooked beef doner kebabs could be
considered a serious public health hazard. These three multiplex PCR assay are a
suitable method for rapid identification of E. coli O157:H7 virulence genes.
undercooked, contaminated meat. In this study, a number of 200 cooked beef
doner kebab samples from different restaurants across cities of Tehran and
Mashhad were analyzed. After enrichment, selective plating and biochemical
tests, the DNA from suspected colonies were extracted. Three multiplex polymerase
chain reaction (PCR) assays to confirm the isolates as E. coli O157:H7 and
the presence of virulence factors were performed. Specific primers for cH7 and
rfbO157 genes in the first and for stx1 and stx2 genes in the second m-PCR were
used. The third m-PCR performed using specific primers for eaeA and EhlyA
genes. From 25 nonsorbitol-fermenting isolates, 21 isolates confirmed as E. coli
O157:H7. The results of third m-PCR showed 12 isolates harboring the EhlyA
gene, three isolates the eaeA gene and three isolates harboring both the EhlyA and
eaeA genes.
PRACTICAL APPLICATIONS
The presence of Escherichia coli O157:H7 in cooked beef doner kebabs could be
considered a serious public health hazard. These three multiplex PCR assay are a
suitable method for rapid identification of E. coli O157:H7 virulence genes.
کلیدواژه(گان): E,coli O157:H7,doner kebab,m-PCR
کالکشن
:
-
آمار بازدید
Isolation, Identification and Virulence Gene Profiling of Escherichia coli O157:H7 in Retail Doner Kebabs, Iran
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| contributor author | Hedayat Hosseini | en |
| contributor author | عبداله جمشیدی | en |
| contributor author | محمدرضا باسامی | en |
| contributor author | Ramin Khaksar | en |
| contributor author | طیبه زینلی | en |
| contributor author | Amin Mousavi Khaneghah | en |
| contributor author | سعید خان زادی | en |
| contributor author | Abdollah Jamshidi | fa |
| contributor author | Mohammad Reza Bassami | fa |
| contributor author | Saeid Khanzadi | fa |
| date accessioned | 2020-06-06T13:15:25Z | |
| date available | 2020-06-06T13:15:25Z | |
| date issued | 2013 | |
| identifier uri | http://libsearch.um.ac.ir:80/fum/handle/fum/3347853 | |
| description abstract | Most illnesses caused by Escherichia coli O157:H7 have been associated with eating undercooked, contaminated meat. In this study, a number of 200 cooked beef doner kebab samples from different restaurants across cities of Tehran and Mashhad were analyzed. After enrichment, selective plating and biochemical tests, the DNA from suspected colonies were extracted. Three multiplex polymerase chain reaction (PCR) assays to confirm the isolates as E. coli O157:H7 and the presence of virulence factors were performed. Specific primers for cH7 and rfbO157 genes in the first and for stx1 and stx2 genes in the second m-PCR were used. The third m-PCR performed using specific primers for eaeA and EhlyA genes. From 25 nonsorbitol-fermenting isolates, 21 isolates confirmed as E. coli O157:H7. The results of third m-PCR showed 12 isolates harboring the EhlyA gene, three isolates the eaeA gene and three isolates harboring both the EhlyA and eaeA genes. PRACTICAL APPLICATIONS The presence of Escherichia coli O157:H7 in cooked beef doner kebabs could be considered a serious public health hazard. These three multiplex PCR assay are a suitable method for rapid identification of E. coli O157:H7 virulence genes. | en |
| language | English | |
| title | Isolation, Identification and Virulence Gene Profiling of Escherichia coli O157:H7 in Retail Doner Kebabs, Iran | en |
| type | Journal Paper | |
| contenttype | External Fulltext | |
| subject keywords | E | en |
| subject keywords | coli O157:H7 | en |
| subject keywords | doner kebab | en |
| subject keywords | m-PCR | en |
| journal title | Journal of Food Safety | fa |
| pages | 489-496 | |
| journal volume | 33 | |
| journal issue | 4 | |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1037777.html | |
| identifier articleid | 1037777 |