Histological Study of Skin Wound Healing with Fish Swim Bladder Matrix

Abstract

Today, because of the variety of wounds, a wide range of wound dressings produce with different<br><br>objectives. One of the wound dressing, that skin tissue engineering scientists were concerned, is the<br><br>use of animal tissue models as biological dressing for wound healing. Since the collagen scaffold has<br><br>many applications in skin tissue engineering, for the first time in this experiment, rutilus fish swimming<br><br>bladder matrix (FSBM) was used as a model of collagen-containing tissue for wound healing in rats. In<br><br>this study, first the FSBM was decellularized by using ionic materials. Then 6 randomly selected rats<br><br>were wounded on the back with 2 wounds 4 mm length. Wounds were divided into two groups: the first<br><br>group as control and the second group using FSBM wound dressing. Wound dressings were changed<br><br>daily. On days 3, 5 and 7 of the start of the experiment, tissue samples were taken from ulcers’ sections<br><br>and wound assessment indicator were evaluated by specific staining criteria. Wound histology image<br><br>analysis showed that the FSBM increased migration of skin fibroblast cells and the process of forming<br><br>the epidermis layer and angiogenesis and finally improved wound healing in compare with the control.<br><br>It is assumed that FSBM is an appropriate model for wound healing and can be used as a new clinical<br><br>biological dressing.