Molecular identification of genotype B, a new genotype ofChlamydophilapsittaci in an African grey parrot(Psittacuserithacus)

Abstract

Objectives: Avian chlamydophilosis is caused by Chlamydiophilapsittaci with the highest infection rates in<br><br>parrots (Psittacidae) and pigeons (Columbiformes). This study was conducted to molecularly characterize<br><br>chlamydophilosisin an African grey parrot and determine its genotype.<br><br>Materials & Methods: A 2 year-old African grey parrot(Psittacuserithacus)was examined because of anorexia,depression, diarrhea, and mild dyspnea.After laboratory tests and radiology of the bird,swabs from choanal cleft and cloaca were collected. DNA extraction and OmpA gene-based diagnostic PCR,using CTU/CTL primers were performed. Finally sequence of the PCR product was compared with sequences obtained from GenBank.<br><br>Results & Conclusion: The established phylogenetic tree based on the genome fragment examined in this report and 12 reference genomes, revealed 100% identity of amplicon sequence with genotype B obtained from previous studies. To the best of our knowledge, this is the first report of genotype B identification from Iran. This study suggests the need for greater awareness of chlamydophilosis in pet bird populations by avian clinicians in Iran.