خواص سمیت سلولی و آنالیز شیمیایی عصاره و فرکشن هایی از قارچ خوراکی دکمه ای

Author:
, , , ,
Year
: 2015
Abstract: So far, few compounds of Agaricus

bisporus (e.g., lectin, unsaturated fatty acids

and polysaccharides) have been identified to

exert in vitro or in vivo anti-cancer activities;

therefore, further research is required to

identify more bioactive compounds. The main

aim of the present study was in vitro evaluation

of the anti-cancer potential of A. bisporus

extracts and fractions. In addition, several

wild-growing species of A. bisporus including

A. devoniensis, A. gennadii and a wild brown

isolate of A. bisporus were investigated

for their possible anti-cancer activities.

Anti-proliferative and cytotoxic properties

of methanol-dichloromethane (1:1) crude

extracts were tested on PC3 and MCF-7 cells.

The results revealed that A. bisporus at 500

μg/ml concentration had significant effects on

PC3 (46%) and MCF-7 (40%) proliferation,

while no significant anti-proliferation was

observed in the extracts of other mushrooms.

Four different fractions of A. bisporus

extracts were separated, using a solvent

polarity gradient in column chromatography.

One of the fractions, eluted with the mixture

of ethyl acetate in petroleum ether (40:60),

exhibited potent anti-proliferative activities,

as well as cytotoxicity in both PC3 and MCF-

7 cells, even at a low concentration (125

μg/ml). Flame ionization detector for gas

chromatography, as well as fourier transform

infrared spectroscopy, showed no fatty acids

in the fractions. The obtained data in this study

illustrated the anti-cancer potential of mycochemicals

present in the mushroom fractions

in vitro. Therefore, the present study indicates

the need for further chemical analysis and

investigations.
Keyword(s): Agaricus Bisporus,Antiproliferation

Chemical Analysis
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