Show simple item record

contributor authorماریا بیهقیen
contributor authorسیدحسن مرعشیen
contributor authorعبدالرضا باقریen
contributor authorمجتبی سنکیانen
contributor authormaria beihaghifa
contributor authorHassan Marashifa
contributor authorAbdolreza Bagherifa
contributor authormojtaba sankianfa
date accessioned2020-06-06T13:37:55Z
date available2020-06-06T13:37:55Z
date issued2018
identifier urihttps://libsearch.um.ac.ir:443/fum/handle/fum/3362741?locale-attribute=fa&show=full
description abstractThe main goal of this study was to investigate the possibility of expressing recombinant protein of C-C chemokine

ligand 21 (CCL21) in Solanum lycopersicum via agroinfiltration. CCL21 is a chemokine can be used for

anti-metastatic of cancer cell lines. To examine the expression of CCL21 protein in S. lycopersicum, the construct

of ccl21 was synthesized. This construct was cloned into pBI121 and the resulting CCL21 plasmid was agroinfiltrated

into S. lycopersicum leaves. Within three days after infiltration, Expression of the foreign gene was

confirmed by quantitative Real-time PCR. A recombinant CCL21 protein was immunogenically detected by

western blot, dot blot and ELISA assay. And results showed that the foreign gene was expressed in the transformed

leaves in high level. Also scratch assay was used to investigate the role of this protein in anti-metastatic

function. The results demonstrated anti-metastatic of cancer cells in the presence of this protein.
en
languageEnglish
titleTransient expression of CCL21as recombinant protein in tomatoen
typeJournal Paper
contenttypeExternal Fulltext
subject keywordsRecombinant vaccine

Agroinfiltration

Tomato

Transient gene expression

Migration

Scratch assay
en
journal titleBiotechnology Reportsfa
pages15-Oct
journal volume5
journal issue17
identifier linkhttps://profdoc.um.ac.ir/paper-abstract-1065965.html
identifier articleid1065965


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record