optimization of Extraction Process of Bioactive Compounds from

Abstract

Bene hull contains antioxidant components.Optimum conditions for bioactive compound extraction<br><br>processes from Bene hull using subcritical water with response surface methodology (RSM) were obtained.Temperature (110-200oC), processing time (30-60 min),and the water to Bene hull ratio (10:1-50:1) were the investigated factors. The optimal conditions for maximizing the antioxidant activity were 196.8oC for 52.6 min and a ratio of 43.6:1 for water to Bene hull. Under these conditions,<br><br>the amount of polyphenolic compounds, the reduction power (RP) (EC50), and the DPPH free radical scavenging activity (RSA) (EC50) were predicted to be 2,284 mg of gallic acid/100 g of Bene hull, 0.2002 mg/mL, and 0.6284 mg/mL, respectively. HPLC analysis was used to identify the main phenolic compounds. The subcritical water extraction technique could be used as a beneficial method<br><br>to obtain bioactive compounds from Bene hull.