Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection
Year
: 1998
Abstract: We report a method for protein labeling, separation by capillary electrophoresis
in a polymer sieving matrix, and detection by laser-induced fluorescence.
Different dyes are used to label standard and sample proteins. A two-spectral
channel detector resolves fluorescence from the sample and standards. Comparison
of the migration time of the sample and standards permits the precise
determination of molecular weight, irrespective of variations in run-to-run
migration times.
in a polymer sieving matrix, and detection by laser-induced fluorescence.
Different dyes are used to label standard and sample proteins. A two-spectral
channel detector resolves fluorescence from the sample and standards. Comparison
of the migration time of the sample and standards permits the precise
determination of molecular weight, irrespective of variations in run-to-run
migration times.
Keyword(s): Sodium dodecyl sulfate,capillary electrophoresis,
proteins,sieving matrix,two-spectral
channel,laser-induced fluorescence detection
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Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection
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| contributor author | محمد حسین احمدزاده | en |
| contributor author | Hossein Ahmadzadeh | fa |
| date accessioned | 2020-06-06T13:12:56Z | |
| date available | 2020-06-06T13:12:56Z | |
| date issued | 1998 | |
| identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3346189 | |
| description abstract | We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times. | en |
| language | English | |
| title | Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection | en |
| type | Journal Paper | |
| contenttype | External Fulltext | |
| subject keywords | Sodium dodecyl sulfate | en |
| subject keywords | capillary electrophoresis | en |
| subject keywords | proteins | en |
| subject keywords | sieving matrix | en |
| subject keywords | two-spectral channel | en |
| subject keywords | laser-induced fluorescence detection | en |
| journal title | Electrophoresis | fa |
| pages | 2175-2178 | |
| journal volume | 19 | |
| journal issue | 4 | |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1034674.html | |
| identifier articleid | 1034674 |