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RISShort communication: Effect of heat stress during the dry period on gene expression in mammary tissue and peripheral blood mononuclear cells
Microarray based gene expression profiling of peripheral blood mononuclear cells in dairy cows with experimental hypocalcemia and milk fever
The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression
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Methods: Using somatic antigens of Ostertagia occidentalis, an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analyzed by real-time quatitative reverse-transcription polymerase...
The effects of Ostertagia occidentalis somatic antigens on ovine TLR2 and TLR4 expression
of Ostertagia occidentalis an important abomasal parasite of ruminants, the expression of ovine TLR2 and TLR4 in peripheral blood mononuclear cells (PBMCs) was analysed by real-time quatitative reverse-transcription polymerase chain reaction (qRT-PCR). Somatic...
Equine bone marrow-derived mesenchymal stem cells: optimization of cell density in primary culture
Background: The primary cell seeding density of bone marrow-derived mononuclear cells (BMMNCs)
affects several cellular behaviors, including attachment to the culture dish, proliferation, and
differentiation.
Methods: The aim...
Hydatid cysts versus ovine innate immunity: can antigens from fertile protoscoleces alter sensor molecules in immune cells
protoscolex antigens, hydatid cyst, ovine neonate, toll-like receptors, peripheral blood mononuclear cells...
Peripheral Blood Mononuclear Cells (PBMC): A useful tool to study expression analysis of molecular markers of Inflammation in human and animal
mediators that coordinate the elimination of pathogens and infected cells. In this study we used peripheral blood mononuclear cells (PBMC) as useful and simple tool to study the molecular markers of inflammation by real time quantitative PCR (qPCR).
Quantification of Expression of Pattern Recognition Receptors (PRRs) in Human Peripheral Blood Mononuclear Cells (PBMC) by Real-Time Quantitative PCR
immunity. In the present study, peripheral blood mononuclear cells (PBMC) of healthy individuals were used to investigate the expression level of TLR2, TLR4, MyD88 and CD14 transcript in these cells. Real-time quantitative PCR was applied to quantify...
Simultaneous Detection of Pattern Recognition Receptors (PRRs) in Human Peripheral Blood Mononuclear Cells (PBMC) by Touchdown PCR
immunity. In the present study, peripheral blood mononuclear cells (PBMC) of healthy individuals were used to investigate the expression of TLR2, TLR4, MyD88 and CD14 transcript in these cells. Touchdown PCR (TD-PCR) was applied to simultaneously detect...