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Cloning, sequencing and expression in Escherichia coli of a lipase gene from Bacillus subtilis DR8806
Year: 2013
Abstract:
Background: Lipases (triacylglycerol ester hydrolases, EC
3.1.1.3) are ubiquitous enzymes that catalyze the hydrolysis
of fats and oils through acting on carboxylic ester bond.
Lipases have potential ...
جداسازی و همسانه سازی ژن لیپاز از باکتری باسیلوس سابتیلیسDR8806 در باکتری E. coli DH5a
Year: 2013
Abstract:
In this research, genomic DNA was prepared from Bacillus subtilisDR8806 which
was previously isolated from Dig Rostam hot spring. Bioinformatic tools were used
to design primers based upon multiple sequence ...
Biochemical characterization and gene cloning of a novel alkaline endo-1-4-glucanase from Bacillus subtilis DR8806
Year: 2016
Abstract:
In the present study, an endo-1-4-glucanase was isolated from Bacillus subtilis DR8806. The enzyme was purified to homogeneity via salt precipitation and ion-exchange chromatography. SDS-PAGE analysis ...