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The effect of short-time microwave exposures on Escherichia coli O157:H7 inoculated onto beef slices

Author:
عبداله جمشیدی
,
حسام الدین سیفی
,
مژگان کوشان
,
Abdollah Jamshidi
,
Hesam A Seifi
,
Mozhgan Kooshan
Year
: 2010
Abstract: Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, hemolyticureamic

syndrom and thrombotic thrombocytopenic purpura. In this study the effect of microwave

irradiation of beef samples which were inoculated with E. coli O157:H7 were investigated. The portions

of fresh beef slices weighting 200 g each and about 10 × 10 × 2 cm in size, were soaked in fully growth

of E. coli O157:H7, in BHI broth. The swab samples were taken from the contaminated samples, after

different times of radiation (10, 20, 30, 40 and 50 s), using a domestic microwave oven at full power. The

bacterial counts were performed by using surface plating on sorbitol Mac Conkey agar supplemented

with cifixime and potassium tellurite. After each experiment the surface temperature of treated samples

were measured. The experiment was carried out in triplicate and it was concluded that the microwave

radiation which enhance the surface temperature more than 70°C, can eliminate the superficial

contamination of cattle beef slices with E. coli O157:H7
URI: https://libsearch.um.ac.ir:443/fum/handle/fum/3402154
Keyword(s): Escherichia coli O157:H7,microwave,beef
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    The effect of short-time microwave exposures on Escherichia coli O157:H7 inoculated onto beef slices

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contributor authorعبداله جمشیدیen
contributor authorحسام الدین سیفیen
contributor authorمژگان کوشانen
contributor authorAbdollah Jamshidifa
contributor authorHesam A Seififa
contributor authorMozhgan Kooshanfa
date accessioned2020-06-06T14:34:02Z
date available2020-06-06T14:34:02Z
date issued2010
identifier urihttps://libsearch.um.ac.ir:443/fum/handle/fum/3402154
description abstractEscherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, hemolyticureamic

syndrom and thrombotic thrombocytopenic purpura. In this study the effect of microwave

irradiation of beef samples which were inoculated with E. coli O157:H7 were investigated. The portions

of fresh beef slices weighting 200 g each and about 10 × 10 × 2 cm in size, were soaked in fully growth

of E. coli O157:H7, in BHI broth. The swab samples were taken from the contaminated samples, after

different times of radiation (10, 20, 30, 40 and 50 s), using a domestic microwave oven at full power. The

bacterial counts were performed by using surface plating on sorbitol Mac Conkey agar supplemented

with cifixime and potassium tellurite. After each experiment the surface temperature of treated samples

were measured. The experiment was carried out in triplicate and it was concluded that the microwave

radiation which enhance the surface temperature more than 70°C, can eliminate the superficial

contamination of cattle beef slices with E. coli O157:H7
en
languageEnglish
titleThe effect of short-time microwave exposures on Escherichia coli O157:H7 inoculated onto beef slicesen
typeJournal Paper
contenttypeExternal Fulltext
subject keywordsEscherichia coli O157:H7en
subject keywordsmicrowaveen
subject keywordsbeefen
journal titleAfrican Journal of Microbiology Researchfa
pages2371-2375
journal volume4
journal issue22
identifier linkhttps://profdoc.um.ac.ir/paper-abstract-1019743.html
identifier articleid1019743
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