Survey on Sulfonamide resistance gene (sul1) in Escherichia coli isolates from broilers in Urmia

Abstract

Objectives:Colibacilosis is one of the most important diseases in poultry industry that causes vast economic<br><br>losses, and it is also dangerous in terms of public health. Antibiotic treatment is the most important tool in dealing<br><br>with the disease. Antimicrobial susceptibility of isolates must be determined before choosing an antibiotic drug.<br><br>This study conducted to evaluate sulfonamide resistance phenotype and genotype of E.coli isolates.<br><br>Material and methods: In this study 44 strains of Escherichia coli were isolated from total of 30 broiler chicken<br><br>flocks of Urmia city and Its sensitivity to five antibiotics disk, including Enrofloxacin, Sulfadiazine, Florfenicol,<br><br>Neomycin, Oxytetracycline were assessed. Polymerase chain reactions were used to identify Sul1 genes.<br><br>Results and conclusion:Antibiogram test results showed that 20 isolates were resistant to sulfadiazine as<br><br>sulfonamide antibiotics. The Resistance rate for each antibiotic was for Sulfadiazine 45.5%, Enrofloxacin 6.8%,<br><br>Oxytetracycline 79.5%, Florfenicol 13.7% Neomycin 0%. In this study Sul1 gene were detected in 25 isolates<br><br>from total 44 isolates of Escherichia coli. The results showed that 5 isolates that have had the Sul1 gene does not<br><br>show antibiotic resistance in antibiogram test. This could indicate a difference in sensitivity of these two tests or<br><br>lack of proper conditions for the gene expression in these five isolates. The results showed that the resistance rate<br><br>of isolates to sulfonamides was high and antibiotic resistance in these two tests, polymerase chain reaction and<br><br>antibiogram, can be varied.