The Evaluation of Amount of TGF-β Produced in Supernatant of Co-culturing of DC Pulsed by Somatic Antigenic Extract of Marshallagiamarshalli and Peripheral T Lymphocyte in People Suffering from Asthma

Abstract

Background: Asthma is a chronic inflammatory disease with high prevalence and socio-economic impacts that causes respiratory dysfunction�According to the hygiene hypothesis due to decreasing of bacterial and parasite infection the prevalence of allergic asthma especially in developed countries is going up�Also dendritic cells have special potential to elect immune responses and ploraizing naive T cells in to different subtypes of helper Tcells� Nowadays DC based immunotherapy is a good alternative approach to treat many disease including cancer� In this study we utilized monocyte derived DC to skew naive T cells in to regulatory T cells by co culturing of DCs with somatic extract of Marshallagiamarshalli in order to treat allergic asthma� Methods: In this study 10 people were chosen, 5 healthy people and 5sick people that affected by asthma� Their blood sample were collected and their PBMCs were isolated and then monocytes separated from lymphocytes by adding of IL-4 and GM-CSF, monocytes differentiate to DC and 4th day, antigen extract of Marshallagiamarshalli added to culture medium �Then at 7th day of culture, these DCs cultured by autologous T cells and finally at 12th day of culture, the supernatants were collected and content of TGF-β were measured by sandwich ELISA� Results: The average amount of TGF-β contents of supernatants in asthma group significantly increased (225±6�1 pq/ml) in comparsion with healthy groups (210±8�5 pq/ml)�(P<0�05) Conclusion: With regarde to hygiene hypothesis in which parasitic worms contain regulatory molecule of immune system, it could be possible producing of tolergenic DCs by antigenic extract of Marshallagiamarshalli� Marshallagiamarshalli and this associated with polarizing of T cells toward regulated T cell in the blood of individuals affected by asthma; by evaluating amount of TGF-β content of supernatant of cell culture, it is clear that DCs could polarize immune responses�