An Investigation On The Mesothelin Characteristics For Immunotoxin Development

Abstract

Cell surface antigens are critical markers for diagnostic and healing of many diseases such as<br><br>cancer. In this regard, mesothelin as specixc antigen, with low expression on the normal cells and<br><br>over-expression on the surface of mesothelioma, is suitable candidate for developing approaches of<br><br>therapeutic and diagnostic of cancer. Bearing in mind, the structural and functional<br><br>characterization as well as the expressing cells of the antigen was considered in this study for<br><br>immunotoxin development. Accordingly, the protein sequence of the mesothelin with the accession<br><br>number ALC62084 were retrieved from NCBI database. PROTEINATLAS were used for assessment the<br><br>expression of the antigen. On the other hand, corresponding specixc ligands of this antigen were<br><br>detected via STRING program. Structural modeling and quality assessment of the models were<br><br>carried out by MODELLER and RAMPAGE programs, respectively. Moreover, topology features of the<br><br>antigen were determined by Mpex program. All data were expressed as mean_standard deviation<br><br>with SPSS 21.0 (SPSS Inc., Chicago, IL, USA). Topology feature of the antigen showed that the C<br><br>terminal of the mesothelin is situating on the extracellular with one transmembrane region.<br><br>Moreover, various expression of the antigen were appeared on the surface of the mesothelial cells.<br><br>However, the highest expression of this antigen was determined on the surface of ovarian cancer<br><br>cells. Furthermore, 3D structure of the antigen showed 98% similarity with appropriate in quality.<br><br>Meanwhile, MUC19, WFDC2, WNT5A, NDUFB5, NDUFB9, MPV17, RALA, LAMTOR3, ZBTB43,<br><br>RNMTL1, HN1 and MOR Ab-009 propounded as corresponding ligands of the antigen with different<br><br>afxnity. In general, the results of this study provide the context for immunotoxins development<br><br>with the capacity to targeting mesothelin on the cell surface of the malignancies.