Molecular identification of genotype B, a new genotype ofChlamydophilapsittaci in an African grey parrot(Psittacuserithacus)
نویسنده:
, , , , , , , , ,سال
: 2016
چکیده: Objectives: Avian chlamydophilosis is caused by Chlamydiophilapsittaci with the highest infection rates in
parrots (Psittacidae) and pigeons (Columbiformes). This study was conducted to molecularly characterize
chlamydophilosisin an African grey parrot and determine its genotype.
Materials & Methods: A 2 year-old African grey parrot(Psittacuserithacus)was examined because of anorexia,depression, diarrhea, and mild dyspnea.After laboratory tests and radiology of the bird,swabs from choanal cleft and cloaca were collected. DNA extraction and OmpA gene-based diagnostic PCR,using CTU/CTL primers were performed. Finally sequence of the PCR product was compared with sequences obtained from GenBank.
Results & Conclusion: The established phylogenetic tree based on the genome fragment examined in this report and 12 reference genomes, revealed 100% identity of amplicon sequence with genotype B obtained from previous studies. To the best of our knowledge, this is the first report of genotype B identification from Iran. This study suggests the need for greater awareness of chlamydophilosis in pet bird populations by avian clinicians in Iran.
parrots (Psittacidae) and pigeons (Columbiformes). This study was conducted to molecularly characterize
chlamydophilosisin an African grey parrot and determine its genotype.
Materials & Methods: A 2 year-old African grey parrot(Psittacuserithacus)was examined because of anorexia,depression, diarrhea, and mild dyspnea.After laboratory tests and radiology of the bird,swabs from choanal cleft and cloaca were collected. DNA extraction and OmpA gene-based diagnostic PCR,using CTU/CTL primers were performed. Finally sequence of the PCR product was compared with sequences obtained from GenBank.
Results & Conclusion: The established phylogenetic tree based on the genome fragment examined in this report and 12 reference genomes, revealed 100% identity of amplicon sequence with genotype B obtained from previous studies. To the best of our knowledge, this is the first report of genotype B identification from Iran. This study suggests the need for greater awareness of chlamydophilosis in pet bird populations by avian clinicians in Iran.
کلیدواژه(گان): Avian Chlamydophilosis,Psittacidae,Columbiformes,African grey parrot,ompA gene
کالکشن
:
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آمار بازدید
Molecular identification of genotype B, a new genotype ofChlamydophilapsittaci in an African grey parrot(Psittacuserithacus)
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| contributor author | اسماء افشاری | en |
| contributor author | جمشید رزم یار | en |
| contributor author | مهدیه زعیمی | en |
| contributor author | مسعود رجبیون | en |
| contributor author | سید علی کارگر | en |
| contributor author | Asma Afshari | fa |
| contributor author | Jamshid Razmyar | fa |
| contributor author | Mahdieh Zaeemi | fa |
| contributor author | Masoud Rajabioun | fa |
| contributor author | Seyyed Ali Kargar | fa |
| date accessioned | 2020-06-06T14:21:11Z | |
| date available | 2020-06-06T14:21:11Z | |
| date copyright | 1/31/2016 | |
| date issued | 2016 | |
| identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3393067 | |
| description abstract | Objectives: Avian chlamydophilosis is caused by Chlamydiophilapsittaci with the highest infection rates in parrots (Psittacidae) and pigeons (Columbiformes). This study was conducted to molecularly characterize chlamydophilosisin an African grey parrot and determine its genotype. Materials & Methods: A 2 year-old African grey parrot(Psittacuserithacus)was examined because of anorexia,depression, diarrhea, and mild dyspnea.After laboratory tests and radiology of the bird,swabs from choanal cleft and cloaca were collected. DNA extraction and OmpA gene-based diagnostic PCR,using CTU/CTL primers were performed. Finally sequence of the PCR product was compared with sequences obtained from GenBank. Results & Conclusion: The established phylogenetic tree based on the genome fragment examined in this report and 12 reference genomes, revealed 100% identity of amplicon sequence with genotype B obtained from previous studies. To the best of our knowledge, this is the first report of genotype B identification from Iran. This study suggests the need for greater awareness of chlamydophilosis in pet bird populations by avian clinicians in Iran. | en |
| language | English | |
| title | Molecular identification of genotype B, a new genotype ofChlamydophilapsittaci in an African grey parrot(Psittacuserithacus) | en |
| type | Conference Paper | |
| contenttype | External Fulltext | |
| subject keywords | Avian Chlamydophilosis | en |
| subject keywords | Psittacidae | en |
| subject keywords | Columbiformes | en |
| subject keywords | African grey parrot | en |
| subject keywords | ompA gene | en |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1055245.html | |
| conference title | The 5th International Veterinary Poultry Congress | en |
| conference location | تهران | fa |
| identifier articleid | 1055245 |