A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk
نویسنده:
, , , , , , ,سال
: 2011
چکیده: Purpose or AIM: The objective of this study was to evaluate the applicability of PCR assay for detection
of cow’s and goat’s milk sold as sheep milk.
Methods and Materials or CASE: Multiplex PCR assay using species specific primers was used to identify
cows’, goats’ and sheep’s somatic cells presented in their milk. In this study 25 milk samples labeled as
\\"sheep milk\\" were purchased from different commercial sources in Mashhad city. After DNA extraction
the samples were examined by the multiplex PCR assay using primers targeting the mitochondrial 12s
and 16s rRNA gene.
RESULTS: The results showed 25% of examined milk samples were proven to be pure sheep milk. Also
25% and 50% of samples were confirmed to be mixed goat and sheep, and cow, goat and sheep milk
respectively.
DISCUSSION: In conclusion, the multiplex PCR assays used in this study can be useful for milk
inspection to detect cow’s and goat’s milk in sheep milk.
of cow’s and goat’s milk sold as sheep milk.
Methods and Materials or CASE: Multiplex PCR assay using species specific primers was used to identify
cows’, goats’ and sheep’s somatic cells presented in their milk. In this study 25 milk samples labeled as
\\"sheep milk\\" were purchased from different commercial sources in Mashhad city. After DNA extraction
the samples were examined by the multiplex PCR assay using primers targeting the mitochondrial 12s
and 16s rRNA gene.
RESULTS: The results showed 25% of examined milk samples were proven to be pure sheep milk. Also
25% and 50% of samples were confirmed to be mixed goat and sheep, and cow, goat and sheep milk
respectively.
DISCUSSION: In conclusion, the multiplex PCR assays used in this study can be useful for milk
inspection to detect cow’s and goat’s milk in sheep milk.
کلیدواژه(گان): Multiplex PCR,Adulteration,Caw Milk,Goat Milk,Sheep Milk
کالکشن
:
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آمار بازدید
A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk
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| contributor author | سعید خان زادی | en |
| contributor author | جمشید رزم یار | en |
| contributor author | عبداله جمشیدی | en |
| contributor author | محمد محسن زاده | en |
| contributor author | Saeid Khanzadi | fa |
| contributor author | Jamshid Razmyar | fa |
| contributor author | Abdollah Jamshidi | fa |
| contributor author | Mohammad Mohsenzadeh | fa |
| date accessioned | 2020-06-06T14:02:10Z | |
| date available | 2020-06-06T14:02:10Z | |
| date copyright | 9/28/2011 | |
| date issued | 2011 | |
| identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3379732 | |
| description abstract | Purpose or AIM: The objective of this study was to evaluate the applicability of PCR assay for detection of cow’s and goat’s milk sold as sheep milk. Methods and Materials or CASE: Multiplex PCR assay using species specific primers was used to identify cows’, goats’ and sheep’s somatic cells presented in their milk. In this study 25 milk samples labeled as \\"sheep milk\\" were purchased from different commercial sources in Mashhad city. After DNA extraction the samples were examined by the multiplex PCR assay using primers targeting the mitochondrial 12s and 16s rRNA gene. RESULTS: The results showed 25% of examined milk samples were proven to be pure sheep milk. Also 25% and 50% of samples were confirmed to be mixed goat and sheep, and cow, goat and sheep milk respectively. DISCUSSION: In conclusion, the multiplex PCR assays used in this study can be useful for milk inspection to detect cow’s and goat’s milk in sheep milk. | en |
| language | English | |
| title | A multiplex polymerase chain reaction for the identification of cows’ and goats’ milk in sheep’s milk | en |
| type | Conference Paper | |
| contenttype | External Fulltext | |
| subject keywords | Multiplex PCR | en |
| subject keywords | Adulteration | en |
| subject keywords | Caw Milk | en |
| subject keywords | Goat Milk | en |
| subject keywords | Sheep Milk | en |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1022261.html | |
| conference title | European Biotechnology Congress 2011 | en |
| conference location | استانبول | fa |
| identifier articleid | 1022261 |