Isolation and detection of salmonella spp. and salmonella enteritidis from broiler carcasses in a poultry abattoir in Mashhad suburb-Iran

Abstract

Introduction: Contaminated poultry meat has been identified as one of the principal food<br><br>borne sources of Salmonella. Conventional culture method for detection of Salmonella<br><br>requires 4-6 days to processing of samples. PCR has become the potential of a powerful<br><br>alternative in microbiological diagnostics due to its rapidity and accuracy. In this<br><br>preliminary study a survey was carried out to determine the prevalence of salmonella spp.<br><br>and its enteritidis serovar in broiler carcasses.<br><br>Matrials and Method: A total of 100 samples, were taken from poultry carcasses which<br><br>were prepared for packaging at a commercial broiler slaughtering facility in Mashhad,<br><br>representing 10 broiler flocks, using rinse test method. Samples were taken using cluster<br><br>sampling method. The presence of salmonella spp. and salmonella enteritidis was assessed<br><br>by pre-enrichment and enrichment of centrifuged rinsed water. Then DNA was extracted<br><br>and multiplex PCR was performed by amplification of invA gene which is specific for<br><br>detection of salmonella spp. and prot6e gene which is specific for detection of salmonella<br><br>enteritidis.<br><br>Results: In this study 14% of poultry carcasses were determined as contaminated with<br><br>salmonella spp. and 6% as contaminated with salmonella enteritidis.<br><br>Conculotion: It is pertinent to use M-PCR in order to provide a more accurate profile of the<br><br>prevalence of salmonella spp and salmonella enteritidis in broiler carcasses that could be<br><br>considered as an appropriate alternative to conventional culture method.