Soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen
Year
: 2009
Abstract: The purpose of the present study was to evaluate ram semen in vitro fertility after the freezing–
thawing process with extenders containing soybean lecithin. Soybean lecithin levels of 0.5, 1 and
2% (w/v) were assessed in combination with 7% glycerol in a basic Tris medium. Bioexcell was
used as control treatment. Semen samples were diluted with extenders and then frozen. The
sperm parameters were assessed after thawing for motility, viability and capacitation status.
Fertility was recorded as cleavage rate at 3day and blastocyst rate at 8day after in vitro
fertilization (IVF). Significant effects of various concentration of soybean lecithin were noted for
the parameters investigated (P≤0.05). The percentage of motility was recorded to be 41.8%,
51.9% and 39.7% for 0.5, 1 and 2% lecithin respectively. Also the percentage of viable
spermatozoa was estimated to be 36.08, 48.06 and 35.7 for 0.5, 1 and 2% lecithin respectively.
Lecithin at 1% had more positive effect than other concentrations. Bioexcell produced 49.18%
percentage of motile spermatozoa and 46.8% viable spermatozoa. No significant differences in
the staining patterns of capacitation status were observed. In IVF experiment, the cleavage rate
being significantly higher in oocytes fertilized with semen cryopreserved in 1% Lecithin.
Different concentration of lecithin had no effect on embryo development. Results indicated that
soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen. Animal origin free
extender based on soybean lecithin we have investigated here is a viable alternative to traditional
egg yolk-based extenders.
thawing process with extenders containing soybean lecithin. Soybean lecithin levels of 0.5, 1 and
2% (w/v) were assessed in combination with 7% glycerol in a basic Tris medium. Bioexcell was
used as control treatment. Semen samples were diluted with extenders and then frozen. The
sperm parameters were assessed after thawing for motility, viability and capacitation status.
Fertility was recorded as cleavage rate at 3day and blastocyst rate at 8day after in vitro
fertilization (IVF). Significant effects of various concentration of soybean lecithin were noted for
the parameters investigated (P≤0.05). The percentage of motility was recorded to be 41.8%,
51.9% and 39.7% for 0.5, 1 and 2% lecithin respectively. Also the percentage of viable
spermatozoa was estimated to be 36.08, 48.06 and 35.7 for 0.5, 1 and 2% lecithin respectively.
Lecithin at 1% had more positive effect than other concentrations. Bioexcell produced 49.18%
percentage of motile spermatozoa and 46.8% viable spermatozoa. No significant differences in
the staining patterns of capacitation status were observed. In IVF experiment, the cleavage rate
being significantly higher in oocytes fertilized with semen cryopreserved in 1% Lecithin.
Different concentration of lecithin had no effect on embryo development. Results indicated that
soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen. Animal origin free
extender based on soybean lecithin we have investigated here is a viable alternative to traditional
egg yolk-based extenders.
Keyword(s): Soybean lecithin,ram semen
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Soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen
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contributor author | M. Sharafi | en |
contributor author | M. H. Nasr-Esfahani | en |
contributor author | N. Nili | en |
contributor author | حسن نصیری مقدم | en |
contributor author | Hassan Nassiri Moghaddam | fa |
date accessioned | 2020-06-06T13:57:27Z | |
date available | 2020-06-06T13:57:27Z | |
date copyright | 8/24/2009 | |
date issued | 2009 | |
identifier uri | https://libsearch.um.ac.ir:443/fum/handle/fum/3376357?locale-attribute=en | |
description abstract | The purpose of the present study was to evaluate ram semen in vitro fertility after the freezing– thawing process with extenders containing soybean lecithin. Soybean lecithin levels of 0.5, 1 and 2% (w/v) were assessed in combination with 7% glycerol in a basic Tris medium. Bioexcell was used as control treatment. Semen samples were diluted with extenders and then frozen. The sperm parameters were assessed after thawing for motility, viability and capacitation status. Fertility was recorded as cleavage rate at 3day and blastocyst rate at 8day after in vitro fertilization (IVF). Significant effects of various concentration of soybean lecithin were noted for the parameters investigated (P≤0.05). The percentage of motility was recorded to be 41.8%, 51.9% and 39.7% for 0.5, 1 and 2% lecithin respectively. Also the percentage of viable spermatozoa was estimated to be 36.08, 48.06 and 35.7 for 0.5, 1 and 2% lecithin respectively. Lecithin at 1% had more positive effect than other concentrations. Bioexcell produced 49.18% percentage of motile spermatozoa and 46.8% viable spermatozoa. No significant differences in the staining patterns of capacitation status were observed. In IVF experiment, the cleavage rate being significantly higher in oocytes fertilized with semen cryopreserved in 1% Lecithin. Different concentration of lecithin had no effect on embryo development. Results indicated that soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen. Animal origin free extender based on soybean lecithin we have investigated here is a viable alternative to traditional egg yolk-based extenders. | en |
language | English | |
title | Soybean lecithin is suitable cryoprotectant for cryopreservation of ram semen | en |
type | Conference Paper | |
contenttype | External Fulltext | |
subject keywords | Soybean lecithin | en |
subject keywords | ram semen | en |
identifier link | https://profdoc.um.ac.ir/paper-abstract-1015605.html | |
conference title | 60th Annual Meeting of the European Federation of Animal Science | en |
conference location | بارسلونا | fa |
identifier articleid | 1015605 |