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contributor authorعبداله جمشیدیen
contributor authorمحمدرضا باسامیen
contributor authorآیدا کسائی رودسریen
contributor authorاعظم محمدیen
contributor authorAbdollah Jamshidifa
contributor authorMohammad Reza Bassamifa
contributor authorAida Kasaei Roodsarifa
contributor authorAzam Mohammadifa
date accessioned2020-06-06T13:56:17Z
date available2020-06-06T13:56:17Z
date copyright8/13/2009
date issued2009
identifier urihttps://libsearch.um.ac.ir:443/fum/handle/fum/3375549?locale-attribute=en&show=full
description abstractThe purpose of this study was to investigate the prevalence of the anaerobic intestinal Brachyspira spp among

laying hens flocks located in Mashhad suburb of Iran. In the first stage, as a pilot study, number of 50 rectal

swabs sample from five laying hen flocks (10 samples from each flock), were collected. Samples were cultured

anaerobically on selective agar and confirmed primarily as intestinal spirochaete by it s spirochaetal form under

phase contrast microscopy. Final confirmation as Brachyspira spp performed by PCR amplification with

specific primers, nested-PCR and finally sequencing the amplified PCR product. The first isolate were used as

positive control during the study. With 4% prevalence of infection in pilot study, the sample size was

determined. In the second stage, number of 130 rectal swab samples from 13 flocks was collected, and

Brachyspira spp were isolated by conventional culture and confirmed by PCR and nested-PCR. The prevalence

of Brachyspira spp infection in laying hens determined as 5%. In this study 16.7% of flocks were determined as

infected with Brachyspira spp. This is the first report of the isolation of Brachyspira spp from laying hens in

Iran. Further studies are needed to clarify the sensitivity and specificity of this test, and also determining the

species and presence of infection in human populations.
en
languageEnglish
titleIsolation and Identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad-Iranen
typeConference Paper
contenttypeExternal Fulltext
subject keywordsBrachyspiraen
subject keywordslaying hensen
subject keywordscultureen
subject keywordsPCRen
identifier linkhttps://profdoc.um.ac.ir/paper-abstract-1014128.html
conference titleششمین همایش ملی بیو تکنولوژیfa
conference locationتهرانfa
identifier articleid1014128


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