Enhanced cutaneous wound healing by the leaf extract of Achillea eriophora D.C. using the in vitro scratch assay

نویسنده:
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سال
: 2017
چکیده: Achillea eriophora D.C. (Asteraceae), an endemic species in Iran, is used extensively

in Iranian folk medicine to treat various ailments, including gastrointestinal disorders, skin

inflammations and wound healing. The present study was aimed to investigate the effects

of the methanolic extract of A. eriophora leaves on the stimulation of human fibroblast

cell proliferation and migration as two pivotal stages in wound healing process.

Methanolic extract was prepared by maceration method. Total phenol and flavonoid

contents were measured using spectrophotometry. Cytotoxic and proliferation effects of

the extract were evaluated by MTT assay on human fibroblast cells. Moreover, migration

of the treated cells was assessed by the closure of a denuded area, made by scratching on the

confluent monolayer cultures. The results of cytotoxicity assay indicated that the

methanolic extract did not have any cytotoxic effect on fibroblast cells when used at

concentrations up to 2 μg/mL. Human fibroblast proliferation was stimulated by low

concentrations of the extract (0.1-0.8 μg/mL), and the highest level of proliferation was

observed in the lowest treatment (0.1 μg/mL). Migration of the cells was induced by

intermediate concentrations (1-30 μg/mL) of the extract and the most effective dose was

estimated at 1 μg/mL. Due to the fact that lower concentration of the extract, which

showed the best proliferation and migration stimulatory effects, were not toxic on the

human fibroblast cells, the crude methanolic extract from the leaves of Achillea eriophora

could be recommended as a potential source for improving the wound healing activity in

skin.
کلیدواژه(گان): Achillea eriophora,Wound healing,Fibroblast cells,Proliferation,Migration
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