Quantitative competitive polymerase chain reaction (QC-PCR) technique is playing an important role in nucleic acid quantification. This paper describes a new statistical approach for data analyzing in relative quantitative ...

Background: Quantitative real time reverse transcription PCR (qRT-PCR) is one of the most important techniques for gene-expression analysis in molecular based studies. Selecting a proper internal control gene for normalizing ...

Two specific characteristics of stem cells, self-renewal and their differentiation capacity have made

them important for vast scientific areas, particularly in the molecular and cell biology of stem cells,

as ...

Background: Bone marrow and adipose tissue are two main sources of mesenchymal stem cells (MSCs). Some of studies suggest that there are some differences in gene expression profile of MSCs-derived from various tissues. To ...

Objective: Adipose tissue is main source for isolation of equine mesenchymal stem cells (MSCs) at different ages. It seems that characteristics of adipose-derived MSCs especially gene expression profile change along with ...

Background: Bone marrow and adipose tissue are the main sources of mesenchymal stem cells (MSCs) for cell-base therapy of musculoskeletal disorders.. It is important to evaluate specific characteristics of these cells and ...

At present, the only proven method for producing sexed semen

in mammals is the cell sorting by flow cytometry, based on

DNA difference. Cell sorting by flow cytometer provides a

powerful tool for ...

Flow cytometry is a widely used application for validating the accuracy of sperm sexing.

However, this method is relatively expensive and requires considerable technical support.

An alternative method employing ...

The rumen anaerobic fungi are primary colonizers of fibrous plant materials that degrade lignin-containing

plant cell walls. With the advancement of molecular enumeration methods, in particular 18S rDNA gene probing ...