Embryonic alkaline phosphatase

(EAP) is expressed during the preimplantation

period of mouse development; however, its

function is unknown. To determine whether the

absence of an EAP gene affects ...

gene knockout (KO) strategies to ablate the CCAT1 gene in different colorectal adenocarcinoma cell lines. We refer to these strategies as “CRISPR excision”, “CRISPR HDR”, and “CRISPR du-HITI”.

Results: In order to obstruct the transcription...