Upon prolonged culture, human embryonic stem (hES) cells undergo adaptation,

exhibiting decreased population doubling times and increased cloning efficiencies, often associated

with karyotypic changes. To ...

RNA. Because of its exquisite

specificity and efficiency, RNAi is being considered as an important tool for gene silencing in living organisms.

Human embryonic stem cells (HESCs) have the ability to both self-renew and differentiate into cell...

We have used RNA interference (RNAi) to downregulate β2-microglobulin and Oct4 in human embryonal carci- noma (hEC) cells and embryonic stem (hES) cells, demonstrating that RNAi is an effective tool for regulat- ing specific ...