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RISIsolation, Culture and Characterization of Chicken Primordial Germ Cells
Nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. Transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. Post-translational changes, especially glycosylation...
Use of Molecular Dynamic Tools in Engineering of Onconase Enzyme to Increase Cellular Uptake and Evade RI
,
fve mutations (K31A, K55A, S72C, R73A and K76A) were selected, because MD results showed that this combination
was efective for the goal of this study. Then, the engineering Onconase was synthesized and cloned into the pET22b (+).
Recombinant protein...
Cloning, Sequencing, and Expression of FanC Antigen of Enterotoxigenic Escherichia Coli
by Ni–NTA column, dot-blotting analysis and SDSPAGE electrophoresis. The concentration of the recombinant protein was calculated as 1.3 mg/ml.
The results of this study showed that E.coli can be used as an appropriate host to produce...
Design and construction of chimeric VP8-S2 antigen for bovine rotavirus and bovine coronavirus
to produce
the recombinant vp8-s2 protein. This recombinant protein may be suitable to investigate to produce
immunoglobulin, recombinant vaccine and diagnostic kit in future studies after it passes biological
activity tests in vivo...
Transient Expression of HA1 Antigen of H5N1 Influenza Virus in Tobacco (Nicotiana tabacum L.) via Agro-infiltration
blotting and demonstrated no discrepancy compared to the positive control (HA1). ELISA
results showed that the HA1 was expressed in the transfected leaves in high level as the yield of
recombinant protein was 8.8 % of TSP and the yield of purified HA1 was 0...
Production and Purification of Specific IgY Against InvG Protein of Salmonella typhimurium
Background: Salmonella infections are one of the most important causes of human and animal diseases, worldwide. InvG gene is
one of the main genes of type III secretion system and leads to initial binding of the bacteria ...
Production of Brucella lumazine Synthase Recombinant Protein to Design a Subunit Vaccine against Undulant Fever
Brucella bacterium causes Brucellosis, an infectious disease spreading from animals to human. Brucella
lumazine synthase (BLS) is a highly immunogenic protein with adjuvant properties, which has been introduced
as ...
Development of An Indirect Enzyme-linked Immunosorbent Assay (ELISA) for the detection and quantification of avian influenza A, subtype H5 using a recombinant H5 antigen expressed in sf9 insect cells
Summary: Avian influenza (AI) is a serious infectious disease caused by negative sense single
strand RNA viruses that belong to the genus influenza virus in the family Orthomyxoviridae. In the
present study, ...
Production and characterization of egg yolk antibody (IgY) against recombinant VP8-S2 antigen
Bovine Rotavirus and Bovine Coronavirus are the most important causes of diarrhea in newborn
calves and in some other species such as pigs and sheep. VP8 subunit of rotavirus is the major
determinant of the ...
In vivo immunogenicity assessment and vaccine efficacy evaluation of a chimeric tandem repeat of epitopic region of OMP31 antigen fused to interleukin 2 (IL-2) against Brucella melitensis in BALB/c mice
of recombinant antigens, IL-2 cytokine as a molecular adjuvant was fused
to recombinant constructs. Recombinant proteins were evaluated for immunological studies in a mouse model (BALB/c).
Results: The results showed that all recombinant proteins could...