Repeated intravenius doses of all-trans-retinoyl B-D-glucuronide is not effectiv in the treatment of
نویسنده:
, , , , , , ,سال
: 2001
چکیده: All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic
acid, has demonstrated high biological activity and low toxicity in most in vitro and in
vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim
was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in
lambs with chronic bacterial bronchopneumonia.
Material/Methods: Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or
Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day
intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 μmol/kg or
2.86–4.42 mg/kg animal body weight) in dimethyl sulfoxide, or dimethyl sulfoxide alone.
Animal behavior and signs of clinical illness were logged daily. Lung and liver samples were
assessed for histopathology, while serum and liver samples were extracted for retinoids and
analyzed by reversed-phase gradient high-performance liquid chromatography.
Results: Repeated injections of highly concentrated all-trans-retinoyl β-D-glucuronide did not cause
changes in appetite, activity or other behaviors nor did it cause histologic lesions in liver and
lung. However, it had no effect on resolution of lung lesions resultant of chronic Mannheimia
haemolytica bronchopneumonia.
Conclusion: Repeated intravenous administration of high amounts of all-trans-retinoyl β-D-glucuronide to
lambs did not elicit signs of gross or microscopic toxicity. However, administering all-transretinoyl
β-D-glucuronide too late in the progression of bacterial pneumonia is thought to be
the main reason for its lack of effect in this model. A retinoid lactone derivative was detected
in sheep serum and liver several days after treatment.
acid, has demonstrated high biological activity and low toxicity in most in vitro and in
vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim
was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in
lambs with chronic bacterial bronchopneumonia.
Material/Methods: Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or
Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day
intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 μmol/kg or
2.86–4.42 mg/kg animal body weight) in dimethyl sulfoxide, or dimethyl sulfoxide alone.
Animal behavior and signs of clinical illness were logged daily. Lung and liver samples were
assessed for histopathology, while serum and liver samples were extracted for retinoids and
analyzed by reversed-phase gradient high-performance liquid chromatography.
Results: Repeated injections of highly concentrated all-trans-retinoyl β-D-glucuronide did not cause
changes in appetite, activity or other behaviors nor did it cause histologic lesions in liver and
lung. However, it had no effect on resolution of lung lesions resultant of chronic Mannheimia
haemolytica bronchopneumonia.
Conclusion: Repeated intravenous administration of high amounts of all-trans-retinoyl β-D-glucuronide to
lambs did not elicit signs of gross or microscopic toxicity. However, administering all-transretinoyl
β-D-glucuronide too late in the progression of bacterial pneumonia is thought to be
the main reason for its lack of effect in this model. A retinoid lactone derivative was detected
in sheep serum and liver several days after treatment.
کلیدواژه(گان): retinoids • RAG • toxicity • bronchopneumonia • intravenous injection • lesions
کالکشن
:
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آمار بازدید
Repeated intravenius doses of all-trans-retinoyl B-D-glucuronide is not effectiv in the treatment of
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| contributor author | Jack M. Gallup | en |
| contributor author | Branka Grubor | en |
| contributor author | Arun B. Barua | en |
| contributor author | غلامرضا محمدی | en |
| contributor author | Kim A. Brogden | en |
| contributor author | James A. Olson | en |
| contributor author | Mark R. Ackermann | en |
| contributor author | Gholam Reza Mohammadi | fa |
| date accessioned | 2020-06-06T14:33:44Z | |
| date available | 2020-06-06T14:33:44Z | |
| date issued | 2001 | |
| identifier uri | http://libsearch.um.ac.ir:80/fum/handle/fum/3401943?locale-attribute=fa | |
| description abstract | All-trans-retinoyl β-D-glucuronide, a water-soluble glucuronic acid conjugate of all-transretinoic acid, has demonstrated high biological activity and low toxicity in most in vitro and in vivo models. Since the reparative effects of retinoids on epithelium are well-known, our aim was to study the effect(s) of intravenously-administered all-trans-retinoyl β-D-glucuronide in lambs with chronic bacterial bronchopneumonia. Material/Methods: Two groups of lambs were inoculated intrabronchially with either pyrogen-free saline or Mannheimia haemolytica. Thirty-three days later, lambs were injected four times at five-day intervals with 2 mL of 116 mM all-trans-retinoyl β-D-glucuronide (6.0–9.3 μmol/kg or 2.86–4.42 mg/kg animal body weight) in dimethyl sulfoxide, or dimethyl sulfoxide alone. Animal behavior and signs of clinical illness were logged daily. Lung and liver samples were assessed for histopathology, while serum and liver samples were extracted for retinoids and analyzed by reversed-phase gradient high-performance liquid chromatography. Results: Repeated injections of highly concentrated all-trans-retinoyl β-D-glucuronide did not cause changes in appetite, activity or other behaviors nor did it cause histologic lesions in liver and lung. However, it had no effect on resolution of lung lesions resultant of chronic Mannheimia haemolytica bronchopneumonia. Conclusion: Repeated intravenous administration of high amounts of all-trans-retinoyl β-D-glucuronide to lambs did not elicit signs of gross or microscopic toxicity. However, administering all-transretinoyl β-D-glucuronide too late in the progression of bacterial pneumonia is thought to be the main reason for its lack of effect in this model. A retinoid lactone derivative was detected in sheep serum and liver several days after treatment. | en |
| language | English | |
| title | Repeated intravenius doses of all-trans-retinoyl B-D-glucuronide is not effectiv in the treatment of | en |
| type | Journal Paper | |
| contenttype | External Fulltext | |
| subject keywords | retinoids • RAG • toxicity • bronchopneumonia • intravenous injection • lesions | en |
| journal title | Medical Science Monitor | fa |
| pages | 345-353 | |
| journal volume | 8 | |
| journal issue | 9 | |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-200348.html | |
| identifier articleid | 200348 |