Ovine corpus luteum expresses leptin mRNA
سال
: 2015
چکیده: Introduction: Leptin hormone an important role in reproduction. It seems that leptin is an important linkage between body metabolism and reproductive system. Moreover, it has been shown that leptin and leptin receptor express in reproductive organs of some species. Hence, laptin has a direct effect on reproduction. Aim of this study was to investigate the expression of leptin mRNA in ovine corpus luteum.
Methods: Ovine ovaries were collected from abattoir and immediately were transported to the laboratory on ice. Corpus luteum pieces sliced and total RNA was extracted and cDNA Synthesis was done. First, PCR reaction was done for β- actin, as a housekeeping gene, to evaluate the accuracy of molecular experiments. PCR reaction was done for leptin using appropriate primers. Fat tissue was used as positive control.
Result: Gel electrophoresis for PCR product was confirmed amplification of 121 bp fragment of leptin. So, it was demonstrated that transcript of leptin is expressed in ovine corpus luteum.
Conclusion: Presence of leptin mRNA in the ovine corpus luteum suggests that leptin is involved in the physiological processes of ovine corpus luteum, which should be clarified.
Methods: Ovine ovaries were collected from abattoir and immediately were transported to the laboratory on ice. Corpus luteum pieces sliced and total RNA was extracted and cDNA Synthesis was done. First, PCR reaction was done for β- actin, as a housekeeping gene, to evaluate the accuracy of molecular experiments. PCR reaction was done for leptin using appropriate primers. Fat tissue was used as positive control.
Result: Gel electrophoresis for PCR product was confirmed amplification of 121 bp fragment of leptin. So, it was demonstrated that transcript of leptin is expressed in ovine corpus luteum.
Conclusion: Presence of leptin mRNA in the ovine corpus luteum suggests that leptin is involved in the physiological processes of ovine corpus luteum, which should be clarified.
کلیدواژه(گان): Leptin mRNA,Ovine,Corpus luteum
کالکشن
:
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آمار بازدید
Ovine corpus luteum expresses leptin mRNA
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contributor author | عباس پرهام | en |
contributor author | مظفر کریم زاده کوشکقاضی | en |
contributor author | Abbas Parham | fa |
contributor author | Mozaffar Karimzade kooshkghazi | fa |
date accessioned | 2020-06-06T14:18:25Z | |
date available | 2020-06-06T14:18:25Z | |
date copyright | 5/23/2015 | |
date issued | 2015 | |
identifier uri | http://libsearch.um.ac.ir:80/fum/handle/fum/3391040 | |
description abstract | Introduction: Leptin hormone an important role in reproduction. It seems that leptin is an important linkage between body metabolism and reproductive system. Moreover, it has been shown that leptin and leptin receptor express in reproductive organs of some species. Hence, laptin has a direct effect on reproduction. Aim of this study was to investigate the expression of leptin mRNA in ovine corpus luteum. Methods: Ovine ovaries were collected from abattoir and immediately were transported to the laboratory on ice. Corpus luteum pieces sliced and total RNA was extracted and cDNA Synthesis was done. First, PCR reaction was done for β- actin, as a housekeeping gene, to evaluate the accuracy of molecular experiments. PCR reaction was done for leptin using appropriate primers. Fat tissue was used as positive control. Result: Gel electrophoresis for PCR product was confirmed amplification of 121 bp fragment of leptin. So, it was demonstrated that transcript of leptin is expressed in ovine corpus luteum. Conclusion: Presence of leptin mRNA in the ovine corpus luteum suggests that leptin is involved in the physiological processes of ovine corpus luteum, which should be clarified. | en |
language | English | |
title | Ovine corpus luteum expresses leptin mRNA | en |
type | Conference Paper | |
contenttype | External Fulltext | |
subject keywords | Leptin mRNA | en |
subject keywords | Ovine | en |
subject keywords | Corpus luteum | en |
identifier link | https://profdoc.um.ac.ir/paper-abstract-1048455.html | |
conference title | International Congress on Reproduction | en |
conference location | تهران | fa |
identifier articleid | 1048455 |