Determination of the Cerebrospinal Fluid Electrolytes Alteration in the Developing Rats Born from Diabetic Mothers

Abstract

Vascular disorders which resulted from diabetes mellitus, could change the balance of electrolytes and water volumes in special vascular systems such as kidney and retinal capillaries. In this research we studied the effect of maternal diabetes on vascular structure of choroid plexus. This effect could imbalance ions transport and CSF homeostasis. Diabetes was induced by streptozotocin (55 mg kg-1), given by a single intraperitoneal injection to femal Wistar rats (250-300 g). Control animals were given an equivalent amount of citrate buffer. CSF was collected directly into a glass micropipette from cisterna magna without previous surgery at 1, 5, 10, 15 days after birth. Then electrolyte concentrations were compared in each two groups by Autoanalyzer method. There is a significant difference (p< 0.01) between electrolytes concentrations in two groups. Diabetes effects on choroid plexus vascular system and possible alterations in permeability of choroid plexus cause changes in CSF volume and composition.