Molecular G typing of bovine rotaviruses in Iran

Abstract

Fifty rotavirus-positive feacal samples, selected from 500 ELISA tested diarrheic specimens were used in this study. Viral RNA was extracted from each sample and reveres transcribed to cDNA. The cDNA was then amplified by oligonucleotide primers specific for RNA segment 9, coding for VP7. After the first amplification, PCR products were subjected to a multiplex semi-nested PCR to investigate the presence of bovine rotavirus serotypes: G6, G8 and G10. The results indicated prevalence of 48 and 26% for G6 and G10 serotypes, respectively. Twenty four percent of the samples showed a mix infection by G6 and G10 serotypes and no sample was found positive for the type G8. With the best of our knowledge this is the first report of molecular typing of bovine rotaviruses in Iran.