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نمایش تعداد 1-9 از 9
Downregulation of miR-21 in HEK293T cells and their conditioned medium in advancement of colorectal cancer treatment
Colorectal cancer as the second leading cause of cancer-related death with high prevalence have attracted scientists to unravel the secret of its underlying mechanisms. Although, novel therapeutics approaches like targeted ...
Construction of the Recombinant Lentiviral Vector Containing Human GH1 Gene and Its Expression in HEK293T Cells
was amplified from a plasmid containing full-length hGH coding sequence and then cloned into the lentiviral vector pCDH-GFP. The HEK293T cells were transduced by the lentivirus particles as a targeted cell. hGH expression status in the recombinant cells were...
Expression and Purification of the Main Component Contained in Camel Milk and Its Antimicrobial Activities Against Bacterial Plant Pathogens
lactoferricin, respectively. Human embryonic kidney 293 (HEK-293) cells were used for synthesizing this recombinant peptide.
Finally, the antibacterial activities of this constructed peptide were investigated under in vitro condition. The result showed that, all...
Evaluation of Oct4 Expression Using p-Oct4-GFP Reporter Construct in Human Embryonic Kidney (HEK293T) Cell line.
fluorescent protein (GFP) under Oct4 promoter for analysing Oct4 expression in the competent cells.
Material & Method:
We used a genetic construct which promote GFP under Oct4 gene promoter and transfected the HEK-293T cells using...
بیان مینی ژنهای فاکتور 9 انعقادی انسانی در سلولهای کلیه انسان
پیشزمینه و هدف: نقص در عملکرد فاکتور 9 منجر به بیماری هموفیلی B میگردد. استفاده از فرآوردههای پلاسمایی جهت جلوگیری از خونریزی، خطر انتقال عوامل عفونی را افزایش میدهد. بنابراین امروزه استفاده از فاکتور 9 نوترکیب، بهعنوان ...
Expression of Enterocin-P in HEK Platform: Evaluation of Its Cytotoxic Effects on Cancer Cell Lines and Its Potency to Interact with Cell-Surface Glycosaminoglycan by Molecular Modeling
anticancer activities have been proved previously. In the present study, Enterocin-P (EntP) as a bacteriocin of E. faecium was
expressed in HEK expression system by pcDNA3.1 + vector. The recombinant peptide was purified from culture medium
using Ni2+
...