Altered patterns of differentiation in karyotypically abnormal human embryonic stem cells
نویسنده:
, , , , , , , ,سال
: 2011
چکیده: Upon prolonged culture, human embryonic stem (hES) cells undergo adaptation,
exhibiting decreased population doubling times and increased cloning efficiencies, often associated
with karyotypic changes. To test whether culture adaptation influences the patterns of
differentiation of hES cells, we compared the expression of genes indicative of distinct embryonic
lineages in the embryoid bodies produced from two early passage, karyotypically normal hES cell
lines, and two late passage, karyotypically abnormal hES cell lines. One of the abnormal lines was
a subline of one of the normal early passage lines. The embryoid bodies from each of the lines
showed evidence of extensive differentiation. However, there were differences in the expression
of several genes, indicating that the culture adapted hES cells show altered patterns of differentiation
compared to karyotypically normal hES cells. The loss of induction of alphafetoprotein in
the culture-adapted cells was especially marked, suggesting that they had a reduced capacity to
produce extra-embryonic endoderm. These changes may contribute to the growth advantages of
genetically variant cells, not only by reflecting an increased tendency to self renewal rather than
to differentiate, but also by reducing spontaneous differentiation to derivatives that themselves
may produce factors that could induce further differentiation of undifferentiated stem cells.
exhibiting decreased population doubling times and increased cloning efficiencies, often associated
with karyotypic changes. To test whether culture adaptation influences the patterns of
differentiation of hES cells, we compared the expression of genes indicative of distinct embryonic
lineages in the embryoid bodies produced from two early passage, karyotypically normal hES cell
lines, and two late passage, karyotypically abnormal hES cell lines. One of the abnormal lines was
a subline of one of the normal early passage lines. The embryoid bodies from each of the lines
showed evidence of extensive differentiation. However, there were differences in the expression
of several genes, indicating that the culture adapted hES cells show altered patterns of differentiation
compared to karyotypically normal hES cells. The loss of induction of alphafetoprotein in
the culture-adapted cells was especially marked, suggesting that they had a reduced capacity to
produce extra-embryonic endoderm. These changes may contribute to the growth advantages of
genetically variant cells, not only by reflecting an increased tendency to self renewal rather than
to differentiate, but also by reducing spontaneous differentiation to derivatives that themselves
may produce factors that could induce further differentiation of undifferentiated stem cells.
کلیدواژه(گان): human embryonic stem cell,differentiation
کالکشن
:
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آمار بازدید
Altered patterns of differentiation in karyotypically abnormal human embryonic stem cells
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| contributor author | ALIREZA FAZELI | en |
| contributor author | CHEE-GEE LIEW | en |
| contributor author | مریم مقدم متین | en |
| contributor author | SARAH ELLIOTT | en |
| contributor author | LAURENT F.C. JEANMEURE | en |
| contributor author | PHILLIP C. WRIGHT | en |
| contributor author | HARRY MOORE | en |
| contributor author | PETER W. ANDREWS | en |
| contributor author | Maryam Moghaddam Matin | fa |
| date accessioned | 2020-06-06T14:35:40Z | |
| date available | 2020-06-06T14:35:40Z | |
| date issued | 2011 | |
| identifier uri | http://libsearch.um.ac.ir:80/fum/handle/fum/3403244 | |
| description abstract | Upon prolonged culture, human embryonic stem (hES) cells undergo adaptation, exhibiting decreased population doubling times and increased cloning efficiencies, often associated with karyotypic changes. To test whether culture adaptation influences the patterns of differentiation of hES cells, we compared the expression of genes indicative of distinct embryonic lineages in the embryoid bodies produced from two early passage, karyotypically normal hES cell lines, and two late passage, karyotypically abnormal hES cell lines. One of the abnormal lines was a subline of one of the normal early passage lines. The embryoid bodies from each of the lines showed evidence of extensive differentiation. However, there were differences in the expression of several genes, indicating that the culture adapted hES cells show altered patterns of differentiation compared to karyotypically normal hES cells. The loss of induction of alphafetoprotein in the culture-adapted cells was especially marked, suggesting that they had a reduced capacity to produce extra-embryonic endoderm. These changes may contribute to the growth advantages of genetically variant cells, not only by reflecting an increased tendency to self renewal rather than to differentiate, but also by reducing spontaneous differentiation to derivatives that themselves may produce factors that could induce further differentiation of undifferentiated stem cells. | en |
| language | English | |
| title | Altered patterns of differentiation in karyotypically abnormal human embryonic stem cells | en |
| type | Journal Paper | |
| contenttype | External Fulltext | |
| subject keywords | human embryonic stem cell | en |
| subject keywords | differentiation | en |
| journal title | International Journal of Developmental Biology | fa |
| pages | 175-180 | |
| journal volume | 55 | |
| journal issue | 2 | |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1022374.html | |
| identifier articleid | 1022374 |