A STUDY ON TOXOPLASMA GONDII AND NEOSPORA CANINUM INFECTION IN SHEEP IN MASHHAD AREA, KHORASAN RAZAVI PROVINCE

نویسنده:
, , ,
سال
: 2015
چکیده: Toxoplasma gondii and Neospora caninum belong to Sarcocystidae

family. Life cycles of them are very similar and they are

capable to infect a wide range of animals in the world. T.

gondii is a main causative of abortion in sheep, while the role

of N. caninum infection in causing abortion in sheep is unknown

and need more investigation. The aim of study was to

compare the rate of T. gondii and N. caninum infection in

sheep in Mashhad area. From February to December 2014,

30 head of sheep were monthly purchased from Torghaba

abattoir. The sheep skull was opened and brain tissue removed

for microscopic and molecular examination. First,

Impression smears were prepared from different parts of

brain areas and stained with Giemsa staining and brain tissue

samples were collected for DNA extraction and bioassay

examination. Specific PCR assay was performed to detect T.

gondii and N. caninum DNA in brain samples. The brain tissues

of PCR-positive samples were bioassayed in the Outbred

Swiss Webster and BALB/C mice. The mice were bled

and tested for T. gondii or N. caninum antibodies 5 weeks p.i

by IFAT. The seropositive mice were also euthanized and

then stained impression smears of brain were prepared and

microscopically examined for tissue cysts observation. In the

present study, the cyst and zoites of T. gondii were not microscopically

observed in any stained smears of brain samples of

sheep. T. gondii DNA was detected in 13 (43%) brain tissues

samples of sheep by nested-PCR. Two groups of inoculated

mice developed antibodies against T. gondii and tissue cysts

were found in the brain smears of some mice of infected

group. N. caninum infection was not detected in any brain

samples of sheep by PCR.Our results show that T. gondii infection

is common in the sheep of Mashhad area and it

seems the N. caninum infection rarely occurs in sheep.
کلیدواژه(گان): Toxoplasma gondii,Neospora caninum,sheep,Mashhad
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