Isolation and Identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad-Iran
نویسنده:
, , , , , , ,سال
: 2009
چکیده: The purpose of this study was to investigate the prevalence of the anaerobic intestinal Brachyspira spp among
laying hens flocks located in Mashhad suburb of Iran. In the first stage, as a pilot study, number of 50 rectal
swabs sample from five laying hen flocks (10 samples from each flock), were collected. Samples were cultured
anaerobically on selective agar and confirmed primarily as intestinal spirochaete by it s spirochaetal form under
phase contrast microscopy. Final confirmation as Brachyspira spp performed by PCR amplification with
specific primers, nested-PCR and finally sequencing the amplified PCR product. The first isolate were used as
positive control during the study. With 4% prevalence of infection in pilot study, the sample size was
determined. In the second stage, number of 130 rectal swab samples from 13 flocks was collected, and
Brachyspira spp were isolated by conventional culture and confirmed by PCR and nested-PCR. The prevalence
of Brachyspira spp infection in laying hens determined as 5%. In this study 16.7% of flocks were determined as
infected with Brachyspira spp. This is the first report of the isolation of Brachyspira spp from laying hens in
Iran. Further studies are needed to clarify the sensitivity and specificity of this test, and also determining the
species and presence of infection in human populations.
laying hens flocks located in Mashhad suburb of Iran. In the first stage, as a pilot study, number of 50 rectal
swabs sample from five laying hen flocks (10 samples from each flock), were collected. Samples were cultured
anaerobically on selective agar and confirmed primarily as intestinal spirochaete by it s spirochaetal form under
phase contrast microscopy. Final confirmation as Brachyspira spp performed by PCR amplification with
specific primers, nested-PCR and finally sequencing the amplified PCR product. The first isolate were used as
positive control during the study. With 4% prevalence of infection in pilot study, the sample size was
determined. In the second stage, number of 130 rectal swab samples from 13 flocks was collected, and
Brachyspira spp were isolated by conventional culture and confirmed by PCR and nested-PCR. The prevalence
of Brachyspira spp infection in laying hens determined as 5%. In this study 16.7% of flocks were determined as
infected with Brachyspira spp. This is the first report of the isolation of Brachyspira spp from laying hens in
Iran. Further studies are needed to clarify the sensitivity and specificity of this test, and also determining the
species and presence of infection in human populations.
کلیدواژه(گان): Brachyspira,laying hens,culture,PCR
کالکشن
:
-
آمار بازدید
Isolation and Identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad-Iran
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| contributor author | عبداله جمشیدی | en |
| contributor author | محمدرضا باسامی | en |
| contributor author | آیدا کسائی رودسری | en |
| contributor author | اعظم محمدی | en |
| contributor author | Abdollah Jamshidi | fa |
| contributor author | Mohammad Reza Bassami | fa |
| contributor author | Aida Kasaei Roodsari | fa |
| contributor author | Azam Mohammadi | fa |
| date accessioned | 2020-06-06T13:56:17Z | |
| date available | 2020-06-06T13:56:17Z | |
| date copyright | 8/13/2009 | |
| date issued | 2009 | |
| identifier uri | http://libsearch.um.ac.ir:80/fum/handle/fum/3375549 | |
| description abstract | The purpose of this study was to investigate the prevalence of the anaerobic intestinal Brachyspira spp among laying hens flocks located in Mashhad suburb of Iran. In the first stage, as a pilot study, number of 50 rectal swabs sample from five laying hen flocks (10 samples from each flock), were collected. Samples were cultured anaerobically on selective agar and confirmed primarily as intestinal spirochaete by it s spirochaetal form under phase contrast microscopy. Final confirmation as Brachyspira spp performed by PCR amplification with specific primers, nested-PCR and finally sequencing the amplified PCR product. The first isolate were used as positive control during the study. With 4% prevalence of infection in pilot study, the sample size was determined. In the second stage, number of 130 rectal swab samples from 13 flocks was collected, and Brachyspira spp were isolated by conventional culture and confirmed by PCR and nested-PCR. The prevalence of Brachyspira spp infection in laying hens determined as 5%. In this study 16.7% of flocks were determined as infected with Brachyspira spp. This is the first report of the isolation of Brachyspira spp from laying hens in Iran. Further studies are needed to clarify the sensitivity and specificity of this test, and also determining the species and presence of infection in human populations. | en |
| language | English | |
| title | Isolation and Identification of Brachyspira pilosicoli from laying hens flocks, using conventional culture and molecular methods in Mashhad-Iran | en |
| type | Conference Paper | |
| contenttype | External Fulltext | |
| subject keywords | Brachyspira | en |
| subject keywords | laying hens | en |
| subject keywords | culture | en |
| subject keywords | PCR | en |
| identifier link | https://profdoc.um.ac.ir/paper-abstract-1014128.html | |
| conference title | ششمین همایش ملی بیو تکنولوژی | fa |
| conference location | تهران | fa |
| identifier articleid | 1014128 |