In vivo effects of allogeneic mesenchymal stem cells in a rat model of acute ischemic kidney injury

Abstract

Objective(s): : Renal ischemia-reperfusion injury (IRI) as a severe condition of acute kidney injury<br><br>(AKI) is the most common clinical problem with high mortality rates of 35-60% deaths in hospital.<br><br>Mesenchymal stem cells (MSC) due to unique regenerative characteristics are ideal candidates for the<br><br>treatment of the ischemic injuries. This work is focused on the administration of MSC to IRI-induced<br><br>AKI Wistar rats and evaluating their significance in AKI treatment.<br><br>Material and Methods: Animals underwent surgical procedure and AKI was induced by 40 min<br><br>bilateral renal pedicle clamping. Immediately after reperfusion, 2×106 rat bone marrow derived MSCs<br><br>were injected via intra-parenchymal or intra-aortic route.<br><br>Results: Animals subjected to AKI after days 1 and 3 showed significant increase in the serum creatinine<br><br>and blood urea nitrogen (BUN) concentration along with a declined glomerular filtration rate (GFR)<br><br>when compared with non-ischemic animals. On the other hand, treated animals showed a significant<br><br>enhanced regeneration as compared to ischemic animals in both administration route groups.<br><br>Conclusion: According to the results concluded from the renoprotective effects of MSC in IRI/AKI,<br><br>MSCs could be considered as promising therapeutic approach for AKI in clinical applications.