Proteomics study reveals the molecular mechanisms underlying water stress tolerance induced by Piriformospora indica in barley

Abstract

Piriformospora indica is amutualistic root endophytic fungus,which transfers several benefits to hosts including enhance plant growth and increase yield under both normal and stress<br><br>conditions. It has been shown that P. indica root-colonization enhances water stress tolerance<br><br>based on general and non-specific plant-species mechanism. To better understand the<br><br>molecular mechanism of P. indica-mediated drought stress tolerance, we designed a set of<br><br>comparative experiments to study the impact of P. indica on barely plants cultivar “Golden<br><br>Promise” grown under different drought levels [Filed capacity (F.C.) and 25% F.C.]. P. indica enhanced root and shoot biomass of colonized plants under both well-watered and<br><br>water-deficit conditions. Proteome analysis of P. indica-colonized barley leaves under<br><br>well-treated and water-deficit conditions resulted in detection of 726 reproducibly protein<br><br>spots.Mass spectrometry analysis resulted in the identification of 45 differentially accumulated<br><br>proteins involved in photosynthesis, reactive oxygen scavenging, metabolisms, signal<br><br>transduction, and plant defense responses. Interestingly, P. indica increased the level of proteins<br><br>involved in photosynthesis, antioxidative defense system and energy transport. We propose<br><br>that P. indica-mediated drought stress tolerance in barely is through photosynthesis<br><br>stimulation, energy releasing and enhanced antioxidative capacity in colonized plants.